antibody rabbit polyclonal anti- prox1  (Covance)

Journal: Development (Cambridge, England)

Article Title: Identifying optimal conditions for precise knock-in of exogenous DNA into the zebrafish genome

doi: 10.1242/dev.204571

Figure Lengend Snippet: Targeted insertion of an ALFA tag using optimized knock-in parameters. (A) Sequence and flanking target insertion sites for foxc1a , prox1a and rasa1a . Left and right homology arms are indicated by magenta and blue, respectively. Black lines denote the Cas9 spacer sequence (line above or below sequence denotes positive or negative strand, respectively) and arrowheads mark the DSB sites. Start or stop codons are underlined. Restriction enzyme sites used to estimate nuclease activity are indicated by boxes and labeled. (B) Camera lucida of a zebrafish embryo at 31 hpf. Reproduced with permission from . (C-E) Whole-mount immunostained embryos imaged by confocal microscopy; lateral views, anterior to the left, dorsal is up. (C) Wild-type embryos immunostained with polyclonal Prox1 antibody. Arrows denote fluorescence in neural tube (nt), lateral line primordium (lat) and muscle pioneers (mp). (D,E) Immunostaining of alfa-prox1a um529 heterozygous (D) and wild-type sibling (E) embryos with a nanobody against ALFA. Scale bar: 80 µm.

Article Snippet: The following antibodies and dilutions were used: sdAb/ALFA-HRP (1:10,000; N1505-HRP, RRID:AB_3075989, NanoTag Biotechnologies), anti-Prox1 polyclonal rabbit antibody (1:1000; 11-002, RRID:AB_10013720, AngioBio) in blocking buffer (PBSTw containing 0.1% Triton X-100/2% DMSO/5% bovine serum albumin/1% goat serum).

Techniques: Knock-In, Sequencing, Activity Assay, Labeling, Confocal Microscopy, Fluorescence, Immunostaining

Journal: Biological Psychiatry Global Open Science

Article Title: 11.7T Diffusion Magnetic Resonance Imaging and Tractography to Probe Human Brain Organoid Microstructure

doi: 10.1016/j.bpsgos.2024.100344

Figure Lengend Snippet: Characterization of 125-day-old brain organoids. (A– E, I , J ) Immunostaining of 10-μm-thick sections of organoids with antibodies recognizing MAP2/TTR (A, B) , FOXG1 (C) , GABA (D) , PROX1 (E) , CTIP2 (I) , and TBR1 ( J ) . (F) Immunostaining of whole organoid for GRIK4. (G, H) Immunostaining of monolayer cultures of neurons generated from differentiating organoids dissociated at day 71 and cultured on Matrigel-coated plate for additional 19 days. Nuclei were counterstained with Hoechst 33342. Scale bar = 250 mm in panels (A–C, I , and J) , 50 μm in panels (D, G, and H) , 10 μm in panel (E), and 100 μm in panel (F) .

Article Snippet: The primary antibodies used were mouse monoclonal anti-β-tubulin III antibody (1:200 dilution, conjugated clone TUJ1; R and D Systems), rabbit polyclonal anti-FOXG1 antibody (1:1000 dilution; Abcam), rabbit polyclonal anti-GABA antibody (1:500 dilution; Sigma-Aldrich), rabbit polyclonal anti-MAP2 antibody (1:500 dilution; EMD Millipore), mouse monoclonal anti-TTR antibody (1:200 dilution; LSBio), rat monoclonal anti-CTIP2 antibody (1:500 dilution; Abcam), rabbit polyclonal anti-TBR1 antibody (1:500 dilution; Abcam), rabbit polyclonal anti-PROX1 antibody (1:200 dilution; Proteintech) and rabbit polyclonal anti-GRIK4 antibody (ThermoFisher, 1:200 dilution).

Techniques: Immunostaining, Generated, Cell Culture

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Leukocyte Chemotactic Receptor Fpr1 Protects Against Aging-related Posterior Subcapsular Cataract Formation

doi: 10.1096/fj.202002135R

Figure Lengend Snippet: (A) Validation of goat anti-Fpr1 antiserum specificity by immunocytochemistry. The cell types analyzed are indicated at the upper left of each panel. HEK, parental or Fpr1-transfected human embryonic kidney 293 cells. Neutrophils are from mice with the indicated genotypes. In panel iii, the antiserum was pre-absorbed with the immunogenic peptide used to raise it. Isotype control antibody staining was negative (not shown). (B) Immunolocalization of Fpr1 in mouse lens. Whole eyes were transverse-sectioned through the optic nerve and pupil. Primary antibodies and mouse genotypes are indicated at the top right of each panel. Control IgG antisera were goat anti-FOXE3 (panel iii) and rabbit anti-PROX1 (panel vi). Anti-FoxE3 stained the cornea but not the lens (not shown), whereas we did not observe anti-PROX1 staining of any ocular structure under the conditions used. Staining was revealed with an appropriate secondary antibody conjugated to Alexa Fluor 594. Orange arrows indicate the anterior lens epithelial monolayer. Nuclei are counterstained blue with DAPI in each image in both A and B. Results are representative of at least 3 independent experiments.

Article Snippet: Polyclonal goat anti-FOXE3 (1:200, Santa Cruz sc-48165) was used as a control for anti-Fpr1 M20, and polyclonal rabbit anti-PROX1 (1:200, Abcam ab38692) was used as a control for anti-FPR1 ab101701.

Techniques: Immunocytochemistry, Transfection, Staining

Journal: Cell reports

Article Title: Stage-specific dynamic reorganization of genome topology shapes transcriptional neighborhoods in developing human retinal organoids

doi: 10.1016/j.celrep.2023.113543

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-PROX1 (1:200) , Abcam , Cat#ab11941; RRID: AB_298722.

Techniques: Recombinant, Modification, DNA Sequencing, Software